Effects of targeted delivery of propionate to the human colon on appetite regulation, body weight maintenance and adiposity in overweight adults

作者：Edward S. Chambers, Alexander Viardot, Arianna Psichas, Douglas J. Morrison, Kevin G. Murphy, Sagen Zac‐Varghese, Kenneth MacDougall, Tom Preston, Catriona M. Tedford, Graham Finlayson, John Edward Blundell, Jimmy David Bell, Elizabeth Louise Thomas, Shahrul Mt‐Isa, Deborah Ashby, Glen R. Gibson, Sofía Kolida, Waljit Singh Dhillo, Stephen Robert Bloom, Wayne Morley, Stuart Clegg, Gary Steven Frost · 发表于：Gut · 年份：2014 · DOI：10.1136/gutjnl-2014-307913 · 被引用次数：1426 · 研究领域：Gut microbiota and health、Protein Hydrolysis and Bioactive Peptides、Diabetes Treatment and Management

OBJECTIVE: The colonic microbiota ferment dietary fibres, producing short chain fatty acids. Recent evidence suggests that the short chain fatty acid propionate may play an important role in appetite regulation. We hypothesised that colonic delivery of propionate would increase peptide YY (PYY) and glucagon like peptide-1 (GLP-1) secretion in humans, and reduce energy intake and weight gain in overweight adults. DESIGN: To investigate whether propionate promotes PYY and GLP-1 secretion, a primary cultured human colonic cell model was developed. To deliver propionate specifically to the colon, we developed a novel inulin-propionate ester. An acute randomised, controlled cross-over study was used to assess the effects of this inulin-propionate ester on energy intake and plasma PYY and GLP-1 concentrations. The long-term effects of inulin-propionate ester on weight gain were subsequently assessed in a randomised, controlled 24-week study involving 60 overweight adults. RESULTS: Propionate significantly stimulated the release of PYY and GLP-1 from human colonic cells. Acute ingestion of 10 g inulin-propionate ester significantly increased postprandial plasma PYY and GLP-1 and reduced energy intake. Over 24 weeks, 10 g/day inulin-propionate ester supplementation significantly reduced weight gain, intra-abdominal adipose tissue distribution, intrahepatocellular lipid content and prevented the deterioration in insulin sensitivity observed in the inulin-control group. CONCLUSIONS: Th...