In Vivo Protein Transduction: Delivery of a Biologically Active Protein into the Mouse

作者：Steven R. Schwarze, Alan L. Ho, Adamina Vocero-Akbani, Steven F. Dowdy · 发表于：Science · 年份：1999 · DOI：10.1126/science.285.5433.1569 · 被引用次数：2430 · 研究领域：RNA Interference and Gene Delivery、Virus-based gene therapy research、CRISPR and Genetic Engineering

Delivery of therapeutic proteins into tissues and across the blood-brain barrier is severely limited by the size and biochemical properties of the proteins. Here it is shown that intraperitoneal injection of the 120-kilodalton beta-galactosidase protein, fused to the protein transduction domain from the human immunodeficiency virus TAT protein, results in delivery of the biologically active fusion protein to all tissues in mice, including the brain. These results open new possibilities for direct delivery of proteins into patients in the context of protein therapy, as well as for epigenetic experimentation with model organisms.