Green fluorescent protein: A perspective

作者：S. James Remington · 发表于：Protein Science · 年份：2011 · DOI：10.1002/pro.684 · 被引用次数：265 · 研究领域：Advanced Fluorescence Microscopy Techniques、Advanced Biosensing Techniques and Applications、Click Chemistry and Applications

A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994-2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ratiometric fluorescent protein biosensors and an overview of the fluorescent proteins derived from coral reef animals. Structure-function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered.