Protective effect of N-glycan bisecting GlcNAc residues on  -amyloid production in Alzheimer's disease

作者：Keiko Akasaka‐Manya, Hiroshi Manya, Yoko Sakurai, Bogusław S. Wójczyk, Yasunori Kozutsumi, Yuko Saito, Naoyuki Taniguchi, Shigeo Murayama, Steven L. Spitalnik, Tamao Endo · 发表于：Glycobiology · 年份：2009 · DOI：10.1093/glycob/cwp152 · 被引用次数：102 · 研究领域：Glycosylation and Glycoproteins Research、Lysosomal Storage Disorders Research、Carbohydrate Chemistry and Synthesis

Alteration of glycoprotein glycans often changes various properties of the target glycoprotein and contributes to a wide variety of diseases. Here, we focused on the N-glycans of amyloid precursor protein whose cleaved fragment, beta-amyloid, is thought to cause much of the pathology of Alzheimer's disease (AD). We previously determined the N-glycan structures of normal and mutant amyloid precursor proteins (the Swedish type and the London type). In comparison with normal amyloid precursor protein, mutant amyloid precursor proteins had higher contents of bisecting GlcNAc residues. Because N-acetylglucosaminyltransferase III (GnT-III) is the glycosyltransferase responsible for synthesizing a bisecting GlcNAc residue, the current report measured GnT-III mRNA expression levels in the brains of AD patients. Interestingly, GnT-III mRNA expression was increased in AD brains. Furthermore, beta-amyloid treatment increased GnT-III mRNA expression in Neuro2a mouse neuroblastoma cells. We then examined the influence of bisecting GlcNAc on the production of beta-amyloid. Both beta-amyloid 40 and beta-amyloid 42 were significantly decreased in GnT-III-transfected cells. When secretase activities were analyzed in GnT-III transfectant cells, alpha-secretase activity was increased. Taken together, these results suggest that upregulation of GnT-III in AD brains may represent an adaptive response to protect them from additional beta-amyloid production.