Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties.

作者：Stephen D. Wolpe, George Davatelis, Barbara A. Sherry, Bruce Beutler, DAVID G. HESSE, Hoai Thi Nguyen, Lyle L. Moldawer, C F Nathan, S. F. Lowry, Anthony Cerami · 发表于：The Journal of Experimental Medicine · 年份：1988 · DOI：10.1084/jem.167.2.570 · 被引用次数：582 · 研究领域：Fatty Acid Research and Health、Adipose Tissue and Metabolism、Neuropeptides and Animal Physiology

We report the identification and purification of a new inflammatory monokine synthesized by the macrophage tumor cell line RAW 264.7 in response to endotoxin. This monokine, which we term "macrophage inflammatory protein" (MIP), is a doublet with an apparent molecular mass of approximately 8,000 daltons on SDS-PAGE but forms aggregates of greater than 2 x 10(6) daltons as assessed by gel filtration. Partial NH2-terminal amino acid sequence data reveal no significant homology with any previously described protein. Although the monokine is anionic under physiological conditions, it is one of two major macrophage-secreted proteins that bind to heparin at high salt concentrations. At 100 ng/ml or greater, MIP is chemokinetic for human polymorphonuclear cells and triggers hydrogen peroxide production. Subcutaneous injection of 10 ng or greater of MIP into footpads of C3H/HeJ mice elicits an inflammatory response, characterized by neutrophil infiltration. These findings suggest that MIP is an endogenous mediator that may play a role in the host responses that occur during endotoxemia and other inflammatory events.