Determination of cholesterol using o-phthalaldehyde

作者：L L Rudel, Mariana Morris · 发表于：Journal of Lipid Research · 年份：1973 · DOI：10.1016/s0022-2275(20)36896-6 · 被引用次数：1049 · 研究领域：Analytical Chemistry and Chromatography、Analytical Methods in Pharmaceuticals、Cholesterol and Lipid Metabolism

A simple, rapid method for the determination of cholesterol in plasma and tissue using o-phthalaldehyde is presented. Comparison of this method with the FeCl(3) method gave identical results. However, the o-phthalaldehyde determination is three times more sensitive than the FeCl(3) determination (molar extinction coefficients of 11,610 and 33,440 for FeCl(3) and o-phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation.