Induction of Apoptosis by IGFBP3 Overexpression in Hepatocellular Carcinoma Cells

作者：Jianjun Han, Dewen Xue, Qiu-Rong Han, Liang Xiao-hong, Li Xie, Sheng Li, Hui-yong Wu, Bao‐Liang Song · 发表于：Asian Pacific Journal of Cancer Prevention · 年份：2015 · DOI：10.7314/apjcp.2014.15.23.10085 · 被引用次数：26 · 研究领域：Growth Hormone and Insulin-like Growth Factors、Cancer, Hypoxia, and Metabolism、Lipid metabolism and disorders

BACKGROUND: The insulin-like growth factor (IGF) system comprises a group of proteins that play key roles in regulating cell growth, differentiation, and apoptosis in a variety of cellular systems. The aim of this study was to investigate the role of insulin-like growth factor binding protein 3 (IGFBP3) in hepatocellular carcinoma. MATERIALS AND METHODS: Expression of IGF2, IGFBP3, and PTEN was analyzed by qRT-PCR. Lentivirus vectors were used to overexpress IGFBP3 in hepatocellular carcinoma cell (HCC) lines. The effect of IGFBP3 on proliferation was investigated by MTT and colony formation assays. RESULTS: Expression of IGF2, IGFBP3, and PTEN in several HCC cell lines was lower than in normal cell lines. After 5-aza-2'-deoxycytidine/trichostatin A treatment, significant demethylation of the promoter region of IGFBP3 was observed in HCC cells. Overexpression of IGFBP3 induced apoptosis and reduced colony formation in HUH7 cells. CONCLUSIONS: Expression of IGF2, IGFBP3, and PTEN in several HCC cell lines was lower than in normal cell lines. After 5-aza-2'-deoxycytidine/ trichostatin A treatment, significant demethylation of the promoter region of IGFBP3 was observed in HCC cells. Overexpression of IGFBP3 induced apoptosis and reduced colony formation in HUH7 cells.