Emergence of NDM-1-producing Klebsiella pneumoniae in Morocco

作者：Laurent Poirel, Amina Benouda, Constantin Hays, Patrice Nordmann · 发表于：Journal of Antimicrobial Chemotherapy · 年份：2011 · DOI：10.1093/jac/dkr384 · 被引用次数：110 · 研究领域：Antibiotic Resistance in Bacteria、Antimicrobial agents and applications、Nosocomial Infections in ICU

OBJECTIVES: To analyse the mechanisms responsible for multidrug resistance in three carbapenem-resistant Klebsiella pneumoniae isolates recovered from two hospitalized patients and an outpatient from the same hospital in Rabat, Morocco. METHODS: PCR and sequencing were used to search for β-lactamase genes. Clonal relationships between the isolates were analysed by multilocus sequence typing and PFGE. RESULTS: A history of prior hospitalization in the same ward was determined for two of the three patients. The three isolates of K. pneumoniae belonged to the same sequence type (ST), ST15, and were clonally related. All three isolates carried the bla(NDM-1) gene and co-expressed the extended-spectrum β-lactamases CTX-M-15 and SHV-5, as well as the narrow-spectrum β-lactamases SHV-1, OXA-1, OXA-9 and TEM-1. The bla(NDM-1) gene was located on an ~250 kb non-typeable plasmid co-harbouring the bla(OXA-1) and bla(CTX-M-15) genes. No link with the Indian subcontinent could be established for the three patients. CONCLUSIONS: This work further emphasizes the spread of NDM-1 producers worldwide.