The DrosDel Deletion Collection: A Drosophila Genomewide Chromosomal Deficiency Resource

作者：Edward J. Ryder, Michael Ashburner, Rosa Bautista-Llácer, Jenny Drummond, Jane Webster, Glynnis Johnson, Terri Morley, Yuk Sang Chan, Fiona M. Blows, Darin Coulson, Günter Reuter, Heiko Baisch, Christian Apelt, Andreas Kauk, Thomas Rudolph, M. Kube, Melanie Klimm, Claudia Nickel, János Szidonya, Péter Maróy, Margit Pál, Åsa Rasmuson-Lestander, Karin M. Ekström, Hugo Stocker, Christoph Hugentobler, Ernst Hafen, David Gubb, Gert O. Pflugfelder, Christian Dörner, Bernard M. Mechler, Heide Schenkel, Joachim Marhold, Florenci Serras, Montserrat Corominas, Adrià Punset, John Roote, Steven Russell · 发表于：Genetics · 年份：2007 · DOI：10.1534/genetics.107.076216 · 被引用次数：233 · 研究领域：Chromosomal and Genetic Variations、Genomics and Phylogenetic Studies、Genomics and Chromatin Dynamics

We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.