In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d‐Amphetamine

作者：Tyra Zetterström, Trevor Sharp, Charles Alexander Marsden, Urban Ungerstedt · 发表于：Journal of Neurochemistry · 年份：1983 · DOI：10.1111/j.1471-4159.1983.tb00893.x · 被引用次数：616 · 研究领域：Neurotransmitter Receptor Influence on Behavior、Pain Mechanisms and Treatments、Neuroscience and Neuropharmacology Research

By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5-hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 X 10(-8) M and 5 X 10(-6) M, respectively. d-Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14-fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively.