Molecular effects of fermented papaya preparation on oxidative damage, MAP Kinase activation and modulation of the benzo[a]pyrene mediated genotoxicity

作者：Okezie I. Aruoma, Renato Colognato, Ilaria Fontana, Joanne Gartlon, Lucia Migliore, Keiko Koike, Sandra Coecke, Evelyn Lamy, Volker Mersch‐Sundermann, Incoronata Laurenza, Luca Benzi, Fumihiko Yoshino, Kyo Kobayashi, Masaichi‐Chang‐il Lee · 发表于：BioFactors · 年份：2006 · DOI：10.1002/biof.5520260205 · 被引用次数：70 · 研究领域：Papaya Research and Applications、Herbal Medicine and Trade Cooperation、Nanoparticles: synthesis and applications

The involvement of oxidative and nitrosative stress mechanisms in several biological and pathological processes including aging, cancer, cardiovascular and neurodegenerative diseases has continued to fuel suggestions that processes can potentially be modulated by treatment with free-radical scavengers and antioxidant. The fermented papaya preparation (FPP) derived from Carica papaya Linn was investigated for its ability to modulate oxidative DNA damage due to H2O2 in rat pheochromocytoma (PC12) cells and protection of brain oxidative damage in hypertensive rats. Cells pre-treated with FPP (50 μg/ml) prior to incubation with H2O2 had significantly increased viability and sustenance of morphology and shape. The human hepatoma (HepG2) cells exposed to H2O2 (50 μM) showed an olive tail moment of 10.56 ± 1.44 compared to 1.37 ± 0.29 of the solvent control. A significant reduction (P ⩽ 0.05) of DNA damage was observed at concentrations ⩾ 10 μg/ml FPP, with 50 μg/ml FPP reducing the genotoxic effect of H2O2 by about 1.5-fold compared to only H2O2 exposed cells. Similarly, concentrations≥50μg/ml FPP significantly reduced DNA migration in co-treated cells compared with only the benzo[a]pyrene treated cells with a dose of 100μg/ml FPP reduced the DNA damage 2-fold. The potential of FPP to regulate the phosphorylation status of ERK 1/2, Akt, and p38 was analyzed by Western blot analysis. FPP showed the potential to modulate the H2O2-induced ERK, Akt and p38 activation with the reduction...