Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells

作者：G Chauvière, M.H. Coconnier, Sophie M. Kernéis, Jacky Fourniat, Alain L. Servin · 发表于：Journal of General Microbiology · 年份：1992 · DOI：10.1099/00221287-138-8-1689 · 被引用次数：249 · 研究领域：Probiotics and Fermented Foods、Proteins in Food Systems、Microbial Metabolites in Food Biotechnology

Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Seven Lactobacillus strains adhered well to the Caco-2 cells, of which three possessed calcium-independent adhesion properties. A high level of calcium-independent adhesion was observed with the human stool isolate Lactobacillus acidophilus strain LB. Scanning electron microscopy revealed that this strain adhered to the apical brush border of the cells. Adhesion increased in parallel with the morphological and functional differentiation of the Caco-2 cells. Two Lactobacillus components were involved in this adhesion. One was protease-resistant and bacterial-surface-associated; the other was heat-stable, extracellular and protease-sensitive.