Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

作者：Marian G. Matushek, Marc J. M. Bonten, Mary K. Hayden · 发表于：Journal of Clinical Microbiology · 年份：1996 · DOI：10.1128/jcm.34.10.2598-2600.1996 · 被引用次数：187 · 研究领域：Genomics and Phylogenetic Studies、Bacterial Identification and Susceptibility Testing、Enterobacteriaceae and Cronobacter Research

A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.