Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

作者：Ning Sun, Nicholas John Panetta, Deepak Mohinder Gupta, Kitchener D. Wilson, Andrew Lee, Fangjun Jia, Shijun Hu, Athena M. Cherry, Robert C. Robbins, Michael Thornton Longaker, Joseph C. Wu · 发表于：Proceedings of the National Academy of Sciences · 年份：2009 · DOI：10.1073/pnas.0908450106 · 被引用次数：511 · 研究领域：Pluripotent Stem Cells Research、CRISPR and Genetic Engineering、Tissue Engineering and Regenerative Medicine

Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.