A Circadian Enhancer Mediates PER-Dependent mRNA Cycling in Drosophila melanogaster

作者：Haiping Hao, David L. Allen, Paul E. Hardin · 发表于：Molecular and Cellular Biology · 年份：1997 · DOI：10.1128/mcb.17.7.3687 · 被引用次数：242 · 研究领域：Circadian rhythm and melatonin、Light effects on plants、Plant Molecular Biology Research

Genes expressed under circadian-clock control are found in organisms ranging from prokaryotes to humans. In Drosophila melanogaster, the period (per) gene, which is required for clock function, is transcribed in a circadian manner. We have identified a circadian transcriptional enhancer within a 69-bp DNA fragment upstream of the per gene. This enhancer drives high-amplitude mRNA cycling under light-dark-cycling or constant-dark conditions, and this activity is per protein (PER) dependent. An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment.