A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation

作者：Fahui Li, Pan Shi, Jiasong Li, Fan Yang, Tianyuan Wang, Wei Zhang, Feng Gao, Wei Ding, Dong Li, Juan Li, Ying Xiong, Jin‐Peng Sun, Weimin Gong, Changlin Tian, Jiangyun Wang · 发表于：Angewandte Chemie International Edition · 年份：2013 · DOI：10.1002/anie.201300463 · 被引用次数：50 · 研究领域：Glycosylation and Glycoproteins Research、RNA and protein synthesis mechanisms、Monoclonal and Polyclonal Antibodies Research

Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein–protein interactions. The highly efficient genetic incorporation of 3,5-difluorotyrosine (F2Y) in E. coli and the use of F2Y as a 19F NMR probe for the tyrosine phosphorylation are reported (see picture).