Magnetic Nanosensors for the Detection of Oligonucleotide Sequences

作者：Lee Josephson, Juan Manuel Sánchez Pérez, Ralph Weissleder · 发表于：Angewandte Chemie International Edition · 年份：2001 · DOI：10.1002/1521-3773(20010903)40:17<3204::aid-anie3204>3.0.co;2-h · 被引用次数：381 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、RNA and protein synthesis mechanisms

Superparamagnetic nanoparticles conjugated to oligonucleotides exert sensitive and reversible effects on the spin-spin relaxation rates of adjacent water protons when the conjugates hybridize to target oligonucleotides (see temperature cycle in the picture). The fluid-phase magnetic sensors provide the basis for detecting specific nucleic acid sequences in turbid media and potentially in tissues in vivo.