RAPID BIOASSAY FOR CLOSTRIDIUM BOTULINUM TYPE-E TOXINS BY INTRAVENOUS INJECTION INTO MICE

作者：Genji Sakaguchi, Sumiko Sakaguchi, Hisashi Kondo · 发表于：Japanese Journal of Medical Science and Biology · 年份：1968 · DOI：10.7883/yoken1952.21.369 · 被引用次数：36 · 研究领域：Botulinum Toxin and Related Neurological Disorders、Clostridium difficile and Clostridium perfringens research、Toxin Mechanisms and Immunotoxins

A bioassay method for Clostridium botulinum type-E toxins has been proposed. The method consists of intravenous injections into mice with serially diluted toxin samples and a reference toxin, determination of the time in minutes from injection to death of each mouse, conversion of the time into an appropriate score, and calculation of the toxicity relative to that of a stable, wellstandardized reference toxin by the parallel-line-assay method. If five mice are injected with each of four to five serial twofold dilutions, the fiducial limits of the toxicity will be about ±27-30%. This method enables us to determine the toxicities of several samples in an hour or two, and the relative value to a reference toxin is highly reproducible. The proposed method to calculate ip LD50 from the death time is applicable only to the trypsin-activated toxin in the undissociated as well as dissociated forms containing about 500 or more ip LD50/ml.