Biochemical characteristics of normal and virally transformed mouse cell lines

作者：Masako Yoshikawa-Fukada, Takayuki Nojima · 发表于：Journal of Cellular Physiology · 年份：1972 · DOI：10.1002/jcp.1040800312 · 被引用次数：22 · 研究领域：RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques、Virus-based gene therapy research

Abstract Normal and virally transformed mouse cells respond differently in vitro to high concentrations of potassium. A higher potassium concentration is required to inhibit multiplication of SV40 transformed 3T3 cells to the same extent as that of normal 3T3 cells. This potassium effect correlates to specific activity of Na‐K dependent ATPase in membranous fraction, normal 3T3 cells having the higher enzyme activity being more sensitive to potassium than SV40‐3T3 cells which have the lower specific activity of the enzyme. Contact inhibition of growth and changes of concanavalin A binding sites which are characteristics of viral transformation influence specific activities of Na‐K dependent ATPase and of adenyl cyclase. Incubation with trypsinized concanavalin A causes SV40‐3T3 to show contact inhibition of growth and at the same time, higher specific activities of both enzymes than the observed in untreated cells. Cellular content of cyclic 3′,5′‐AMP of contact inhibited 3T3 is higher than that in transformed SV40‐3T3, but no difference is detectable in ATP content.