Life without DNA repair

作者：David M. Wilson, Larry H. Thompson · 发表于：Proceedings of the National Academy of Sciences · 年份：1997 · DOI：10.1073/pnas.94.24.12754 · 被引用次数：234 · 研究领域：DNA Repair Mechanisms、PARP inhibition in cancer therapy、CRISPR and Genetic Engineering

The advent of gene targeting techniques has permitted the construction of specific genetic deficiencies to evaluate the biological contribution(s) of an individual protein. Mice lacking a precise DNA repair activity have been generated, and these mutants show various combinations of defective embryogenesis, tissue-specific dysfunction, hypersensitivity to DNA-damaging agents, premature senescence, genetic instability, and elevated cancer rates (1). That repair-deficient animals display such abnormalities underscores the fundamental importance of DNA repair in protecting against the mutagenic and cytotoxic effects of DNA damage.