A New Method for the Preparation of Acyl-CoA Thioesters1

作者：Akihiko Kawaguchi, Tsutomu YOSHlMURA, Shigenobu Okuda · 发表于：The Journal of Biochemistry · 年份：1981 · DOI：10.1093/oxfordjournals.jbchem.a133207 · 被引用次数：173 · 研究领域：Phosphorus compounds and reactions

A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (from C2 to C18) and with varying degrees of unsaturation. It is particularly suitable for preparing acyl-CoA thioesters of labeled fatty acids or fatty acids available only in small amounts.