Structure of PTB Bound to RNA: Specific Binding and Implications for Splicing Regulation

作者：Florian C. Oberstrass, Sigrid Auweter, Michèle C. Erat, Yann Hargous, A Henning, Philipp Wenter, Luc Reymond, Batoul Amir-Ahmady, Stefan Pitsch, Douglas L. Black, Frédéric H.‐T. Allain · 发表于：Science · 年份：2005 · DOI：10.1126/science.1114066 · 被引用次数：477 · 研究领域：RNA Research and Splicing、RNA and protein synthesis mechanisms、RNA modifications and cancer

The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.