Airborne spread and infection of a novel swine-origin influenza A (H1N1) virus

作者：Hongna Zhang, Xin Li, Ruihua Ma, Xiaoxia Li, Yufa Zhou, Hongliang Dong, Xinxian Li, Qinglei Li, Mingliang Zhang, Zhihao Liu, Baozhi Wei, Mingchao Cui, Hao Wang, Jing Gao, Huili Yang, Peiqiang Hou, Zengmin Miao, Tongjie Chai · 发表于：Virology Journal · 年份：2013 · DOI：10.1186/1743-422x-10-204 · 被引用次数：43 · 研究领域：Influenza Virus Research Studies、Infection Control and Ventilation、Respiratory viral infections research

BACKGROUND: The novel swine-origin influenza A (H1N1) virus (S-O 2009 IV) can cause respiratory infectious diseases in humans and pigs, but there are few studies investigating the airborne spread of the virus. In January 2011, a swine-origin H1N1 epidemic emerged in eastern China that rapidly spread to neighboring farms, likely by aerosols carried by the wind. METHODS: In this study, quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect viruses in air samples from pig farms. Based on two aerosol infection models (Pig and guinea pig), we evaluated aerosol transmission and infection of the novel S-O 2009 IV isolate. RESULTS: Three novel S-O 2009 IV were isolated from the diseased pig. The positive rate and viral loads of air samples were 26.1% and 3.14-5.72 log₁₀copies/m³ air, respectively. In both pig and guinea pig infection models, the isolate (A/swine/Shandong/07/2011) was capable of forming aerosols and infected experimental animals at a range of 2.0-4.2 m by aerosols, but aerosol route was less efficient than direct contact. CONCLUSIONS: The results indicated that S-O 2009 IV is able to be aerosolized by infected animals and to be transmitted to susceptible animals by airborne routes.