Expression profiling and integrative analysis of the CESA/CSL superfamily in rice

作者：Lingqiang Wang, Kai Guo, Yu Li, Yuanyuan Tu, Huizhen Hu, Bingrui Wang, Xiaocan Cui, Liangcai Peng · 发表于：BMC Plant Biology · 年份：2010 · DOI：10.1186/1471-2229-10-282 · 被引用次数：292 · 研究领域：Polysaccharides and Plant Cell Walls、Advanced Cellulose Research Studies、Plant nutrient uptake and metabolism

BACKGROUND: The cellulose synthase and cellulose synthase-like gene superfamily (CESA/CSL) is proposed to encode enzymes for cellulose and non-cellulosic matrix polysaccharide synthesis in plants. Although the rice (Oryza sativa L.) genome has been sequenced for a few years, the global expression profiling patterns and functions of the OsCESA/CSL superfamily remain largely unknown. RESULTS: A total of 45 identified members of OsCESA/CSL were classified into two clusters based on phylogeny and motif constitution. Duplication events contributed largely to the expansion of this superfamily, with Cluster I and II mainly attributed to tandem and segmental duplication, respectively. With microarray data of 33 tissue samples covering the entire life cycle of rice, fairly high OsCESA gene expression and rather variable OsCSL expression were observed. While some members from each CSL family (A1, C9, D2, E1, F6 and H1) were expressed in all tissues examined, many of OsCSL genes were expressed in specific tissues (stamen and radicles). The expression pattern of OsCESA/CSL and OsBC1L which extensively co-expressed with OsCESA/CSL can be divided into three major groups with ten subgroups, each showing a distinct co-expression in tissues representing typically distinct cell wall constitutions. In particular, OsCESA1, -3 & -8 and OsCESA4, -7 & -9 were strongly co-expressed in tissues typical of primary and secondary cell walls, suggesting that they form as a cellulose synthase complex; thes...