The Genome of the Basidiomycetous Yeast and Human Pathogen Cryptococcus neoformans

作者：Brendan Loftus, Eula Fung, Paola Roncaglia, Don Rowley, Paolo Amedeo, Dan Bruno, Jessica Vamathevan, Molly Miranda, Iain Anderson, James A. Fraser, Jonathan Allen, Ian E. Bosdet, Michael R. Brent, Readman Chiu, Tamara L. Doering, Maureen J. Donlin, Cletus A. D’Souza, Deborah S. Fox, V.B. Grinberg, Jianmin Fu, Marilyn Fukushima, Brian J. Haas, James Huang, Guilhem Janbon, Steven J.M. Jones, Hyunwoo Koo, Martin Krzywinski, June K. Kwon-Chung, Klaus B. Lengeler, Rama Maiti, Marco A. Marra, Robert E. Marra, Carrie Mathewson, Thomas G. Mitchell, Mihaela Pertea, Florenta Riggs, Steven L. Salzberg, Jacqueline E. Schein, Alla Shvartsbeyn, Heesun Shin, Martin Shumway, Charles A. Specht, Bernard Suh, Aaron Tenney, Terry Utterback, Brian L. Wickes, Jennifer R. Wortman, Natasja H. Wye, James W. Kronstad, Jennifer K. Lodge, Joseph Heitman, Ronald W. Davis, Claire M. Fraser, Richard W. Hyman · 发表于：Science · 年份：2005 · DOI：10.1126/science.1103773 · 被引用次数：727 · 研究领域：Yeasts and Rust Fungi Studies、Fungal Infections and Studies、Plant Disease Resistance and Genetics

Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its approximately 20-megabase genome, which contains approximately 6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.