Catabolite inactivation of the yeast maltose transporter occurs in the vacuole after internalization by endocytosis

作者：E Riballo, Marga Herweijer, Detlef Wolf, Rosario Lagunas · 发表于：Journal of Bacteriology · 年份：1995 · DOI：10.1128/jb.177.19.5622-5627.1995 · 被引用次数：93 · 研究领域：Fungal and yeast genetics research、Cellular transport and secretion、Metabolism, Diabetes, and Cancer

The maltose transporter of Saccharomyces cerevisiae is rapidly degraded during fermentation in the absence of a nitrogen source. The location and mechanism of degradation of the transporter have been investigated. Using mutants defective in endocytosis, we have shown that degradation of this transporter requires internalization by endocytosis. In addition, studies of mutants defective in proteasome or vacuolar proteolysis revealed that degradation occurs in the vacuole and is independent of proteasome function. The results also revealed that degradation of the maltose transporter requires Sec18p and raised the question of whether in the absence of Sec18p activity the internalized maltose transporter is recycled back to the plasma membrane.