Effect of tumor necrosis factor alpha on mitogen-activated human B cells.

作者：John H. Kehrl, ANNETTE R. MILLER, Anthony S. Fauci · 发表于：The Journal of Experimental Medicine · 年份：1987 · DOI：10.1084/jem.166.3.786 · 被引用次数：237 · 研究领域：Neuropeptides and Animal Physiology、Receptor Mechanisms and Signaling、Monoclonal and Polyclonal Antibodies Research

In this study we demonstrate that the monocyte/macrophage product, tumor necrosis factor alpha (TNF-alpha), has significant in vitro effects of B cell function. It costimulated with anti-mu in the induction of B cell DNA synthesis, and it prolonged the DNA synthesis initiated in B cell cultures stimulated with the human B cell mitogen, Staphylococcus aureus Cowan strain I (SAC). The addition of either IL-1 or IFN-gamma to TNF-alpha resulted in a substantial further increase in DNA synthesis. The addition of TNF-alpha to IL-2, a known inducer of SAC-activated B cell Ig secretion, resulted in a twofold enhancement in the amount of IL-2 stimulated B cell Ig secretion. Receptor binding studies with 125I-TNF-alpha demonstrate a marked increase in TNF-alpha binding sites after B cell activation (approximately 6,000 sites per cell, with an apparent Kd of 2.0 X 10(-10) M). Thus, TNF-alpha may be an important factor in human B cell function and is likely to interact with other T cell and monocyte derived cytokines in the regulation of human B cell proliferation and Ig production.