Molecular characterization of two novel C-type lectin-like receptors, one of which is selectively expressed in human dendritic cells

作者：Marco Colonna, Jacqueline Samaridis, Lena Ångman · 发表于：European Journal of Immunology · 年份：2000 · DOI：10.1002/1521-4141(200002)30:2<697::aid-immu697>3.0.co;2-m · 被引用次数：219 · 研究领域：Immune Cell Function and Interaction、Immunotherapy and Immune Responses、Invertebrate Immune Response Mechanisms

We have identified two human C-type lectin-like receptors, CLEC-1 and CLEC-2. Both display a single carbohydrate recognition domain and a cytoplasmic tyrosine-based motif. They are homologous to the NK cell receptors NKG2s and CD94 and also to the oxidized low-density lipoprotein receptor 1. CLEC-1 and CLEC-2 are preferentially transcribed in dendritic cells (DC) and in the liver, respectively. Following transient transfection in COS cells, CLEC-1 is expressed intracellularly, perhaps requiring an associated chain to reach the cell surface. CLEC-2 is expressed on the surface of transfected cells as a protein of approximately 33 kDa. CLEC-1 and CLEC-2 genes map to human chromosome 12, most likely in linkage with the NK gene complex (NKC). Thus, the NKC may encode C-type lectin-like receptors expressed not only in NK cells but also in other cells, and at least one of these is of potential importance in regulating DC function.