The Polypeptide Composition of Isolated Surface Projections of Avian Infectious Bronchitis Virus

作者：Michael Collins, D.J. Alexander · 发表于：Journal of General Virology · 年份：1980 · DOI：10.1099/0022-1317-48-1-213 · 被引用次数：10 · 研究领域：Animal Virus Infections Studies、Bacteriophages and microbial interactions、Viral gastroenteritis research and epidemiology

Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 M-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 M-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86,000 and 66,000 mol. wt. The larger polypeptide was glycosylated.