Rapid Increase in Adenosine 5′-Triphosphate during Early Wheat Embryo Germination

作者：Ralph L. Obendorf, Abraham Marcus · 发表于：PLANT PHYSIOLOGY · 年份：1974 · DOI：10.1104/pp.53.5.779 · 被引用次数：66 · 研究领域：Plant Micronutrient Interactions and Effects

The ATP content of isolated wheat (Triticum aestivum L. var. Polk) embryos increases 5-fold during the first 30 minutes and 10-fold during the first hour of germination to 80% of maximum. The ATP level remains at approximately 800 nanomoles per gram of tissue during the next 15 hours. ADP, AMP, and total adenosine phosphates decrease between 1 and 6.5 hours, while adenylate energy charge increases from 0.6 to 0.8 and remains constant. The rapid increase in ATP during imbibition is consistent with the energy requirement for polyribosome formation and protein synthesis during the first hours of germination. A method for determining nanomole quantities of ATP in tissue extracts by isotopic dilution of gamma-(32)P-ATP in the hexokinase reaction is outlined.