STUDIES ON COPPER METABOLISM. XIV. COPPER, CERULO-PLASMIN AND OXIDASE ACTIVITY IN SERA OF NORMAL HUMAN SUBJECTS, PREGNANT WOMEN, AND PATIENTS WITH INFECTION, HEPATOLENTICULAR DEGENERATION AND THE NEPHROTIC SYNDROME 1

作者：Harold Markowitz, Clark J. Gubler, John P. Mahoney, G. E. Cartwright, Maxwell M. Wintrobe · 发表于：Journal of Clinical Investigation · 年份：1955 · DOI：10.1172/jci103201 · 被引用次数：235 · 研究领域：Trace Elements in Health、Heavy Metal Exposure and Toxicity、Selenium in Biological Systems

Ceruloplasmin, the blue copper-containing pro- tein of serum, was first isolated in 1948 by Holm- berg and Laurell (1).They characterized this protein as an alpha 2 globulin with a copper con- tent of approximately 0.32 per cent,3 a molecular weight of about 151,000 and 8 atoms of copper per molecule.The substance has been shown to exhibit oxidase activity with a variety of sub- strates.The enzyme is most active with para- phenylene diamine (2, 3).Holmberg and Laurell (4) observed that ceru- loplasmin could be precipitated from normal hu- man serum made 50 per cent saturated with am- monium sulfate.However, approximately 2 to 12 per cent of the total copper remained in the solu- ble "albumin" fraction.They suggested that the copper remaining in the albumin fraction might be attached to globulin which could not be com- pletely separated from the albumin by ammonium sulfate precipitation.More recently, in our laboratory (5), it was demonstrated that there is a small fraction of the plasma copper (0 to 17 per cent) in normal subjects which reacts directly