Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.

作者：Hazel L. Sive, Robert G. Roeder · 发表于：Proceedings of the National Academy of Sciences · 年份：1986 · DOI：10.1073/pnas.83.17.6382 · 被引用次数：201 · 研究领域：RNA Research and Splicing、RNA and protein synthesis mechanisms、Genomics and Chromatin Dynamics

We have examined the interaction of factors in HeLa cell nuclear extracts with a human histone H2B gene (H2B) promoter. Protein-DNA mobility-shift and DNase I protection assays detected a factor(s) binding to a 15-base-pair consensus element that is essential for efficient H2B transcription in vitro. Part of this consensus sequence is the octanucleotide ATTTGCAT, which is apparently a functional component of several non-histone genes. A subset of these genes, including a human U2 small nuclear RNA (snRNA) gene promoter, a mouse immunoglobulin heavy chain enhancer, and a mouse light chain promoter, were shown to interact with the H2B consensus sequence-binding factor(s). These results suggest that a common factor or closely related factors may contribute to the regulation of these and other genes that share the octanucleotide sequence.