Surface Co-Expression of Two Different PfEMP1 Antigens on Single Plasmodium falciparum-Infected Erythrocytes Facilitates Binding to ICAM1 and PECAM1

作者：Louise Joergensen, Dominique C. Bengtsson, Anja Bengtsson, Elena Ronander, Sanne Schou Berger, Louise Turner, Michael B. Dalgaard, Gerald K. K. Cham, Michala E. Victor, Thomas Lavstsen, Thor Grundtvig Theander, David E. Arnot, Anja T. R. Jensen · 发表于：PLoS Pathogens · 年份：2010 · DOI：10.1371/journal.ppat.1001083 · 被引用次数：133 · 研究领域：Malaria Research and Control、Complement system in diseases、Drug Transport and Resistance Mechanisms

The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens play a major role in cytoadhesion of infected erythrocytes (IE), antigenic variation, and immunity to malaria. The current consensus on control of variant surface antigen expression is that only one PfEMP1 encoded by one var gene is expressed per cell at a time. We measured var mRNA transcript levels by real-time Q-PCR, analysed var gene transcripts by single-cell FISH and directly compared these with PfEMP1 antigen surface expression and cytoadhesion in three different antibody-selected P. falciparum 3D7 sub-lines using live confocal microscopy, flow cytometry and in vitro adhesion assays. We found that one selected parasite sub-line simultaneously expressed two different var genes as surface antigens, on single IE. Importantly, and of physiological relevance to adhesion and malaria pathogenesis, this parasite sub-line was found to bind both CD31/PECAM1 and CD54/ICAM1 and to adhere twice as efficiently to human endothelial cells, compared to infected cells having only one PfEMP1 variant on the surface. These new results on PfEMP1 antigen expression indicate that a re-evaluation of the molecular mechanisms involved in P. falciparum adhesion and of the accepted paradigm of absolutely mutually exclusive var gene transcription is required.