THE INCORPORATION OF PLASMA FREE FATTY ACIDS INTO PLASMA TRIGLYCERIDES IN MAN*

作者：S. J. Friedberg, Robert F. X. Klein, David L. Trout, Morton D. Bogdonoff, E. Harvey Estes · 发表于：Journal of Clinical Investigation · 年份：1961 · DOI：10.1172/jci104409 · 被引用次数：188 · 研究领域：Adipose Tissue and Metabolism、Fatty Acid Research and Health、Muscle metabolism and nutrition

Plasma triglycerides (neutral fat) may be di- vided into 1) that portion which is present during the fasting state and 2) that which, after ingestion of fat, is added to and temporarily augments the fasting level of triglycerides.The present investigation deals with the rela- tionship of plasma free fatty acids (plasma FFA) to fasting plasma triglycerides (plasma TG) in man.From these observations a quantitative as- sessment of the amount of plasma FFA converted to plasma TG will be presented.The data also permit calculations which suggest that plasma FFA may be the major source of fasting plasma TG. METHODSTwo types of studies were carried out in male patients without known abnormalities of fat metabolism.1. Non-reinjection experiments.After an overnight fast of 15 hours, 9 subjects were given 0.004 to 0.008 mc albumin-bound palmitic acid-i-C1' intravenously either by single injection or by 2-hour constant infusion.Two individuals received 0.00585 mc of albumin-bound oleic acid-i-C1' and another 0.00597 mc of stearic acid-1-C14.During the day of the study the subjects were permitted no breakfast, a nonfat lunch, and an unrestricted supper.Venous blood samples were drawn at appropriate in- tervals over a period of 24 hours after administration of fatty acid C1' for determination of neutral fat activity.Two-ml aliquots of plasma were extracted by the method of Dole (1).Eight ml of the upper phase, representing 1.85 ml of plasma, was transferred to a separate tube.Ten ml of 0.1...