Kinetics of branch migration in double-stranded DNA.

作者：B. J. Thompson, Merrill N. Camien, R C Warner · 发表于：Proceedings of the National Academy of Sciences · 年份：1976 · DOI：10.1073/pnas.73.7.2299 · 被引用次数：135 · 研究领域：Bacteriophages and microbial interactions、DNA and Nucleic Acid Chemistry、Nanopore and Nanochannel Transport Studies

The rate of branch migration in double stranded DNA has been measured by the use of a unique substrate formed by the action of the EcoRI restriction endonuclease on the dimeric figure-8 configuration of the replicative form DNA of phage G4. The figure-8 and the X-form derived from it contain a junction of the kind postulated to occur in the Holliday structure and to be an essential feature of a number of models of recombination. In the X-form this junction can branch migrate to an irreversible terminal configuration consisting of two linear monomers. The disappearance of X-forms was measured by electron microscopy. A treatment of branch migration as a random walk process was developed to permit the determination of the rate of the intrinsic process, a step movement of the junction by a distance of one base pair. A value of about 6 kilobase pairs per sec at 37 degrees was obtained.