Use of N-terminal modified poly(L-lysine)-antibody conjugate as a carrier for targeted gene delivery in mouse lung endothelial cells

作者：Vladimir S. Trubetskoy, Vladimir P. Torchilin, Stephen J. Kennel, Leaf Huang · 发表于：Bioconjugate Chemistry · 年份：1992 · DOI：10.1021/bc00016a011 · 被引用次数：134 · 研究领域：RNA Interference and Gene Delivery、Viral Infectious Diseases and Gene Expression in Insects、Microfluidic and Bio-sensing Technologies

A DNA targeted delivery and expression system has been designed based on an N-terminal modified poly(L-lysine) (NPLL)-antibody conjugate, which readily forms a complex with plasmid DNA. Monoclonal antibodies against the cell-surface thrombomodulin conjugated with NPLL were used for targeted delivery of foreign plasmid DNA to an antigen-expressing mouse lung endothelial cell line in vitro and to mouse lungs in vivo. In both cases significant amounts of DNA can be specifically bound to the target cells or tissues. Specific gene expression was observed in the treated mouse lung endothelial cells.