Mapping of mutations affecting pyoverdine production inPseudomonas aeruginosa

作者：Dany Hohnadel, Dieter Haas, Jean‐Marie Meyer · 发表于：FEMS Microbiology Letters · 年份：1986 · DOI：10.1111/j.1574-6968.1986.tb01695.x · 被引用次数：91 · 研究领域：Bacterial Genetics and Biotechnology、Plant Pathogenic Bacteria Studies、Genomics and Phylogenetic Studies

Pyoverdine, the yellow-green fluorescent pigment produced by Pseudomonas aeruginosa, is a highly efficient siderophore. Pyoverdine-deficient (pvd) mutants of P. aeruginosa PAO isolated after mutagenesis were non-fluorescent and unable to grow in the presence of 2.8 mM ethylenediamine-di-(o-hydroxyphenylacetate) (EDDHA). Addition of purified pyoverdine to media containing EDDHA restored growth of pvd mutants. 6 pvd mutations were mapped between catA and mtu-9002 (at 65–70 min on the chromosome map) by R68.45-mediated conjugation. 2 slightly leaky pvd mutations were localised between argC and strA (at 35 min) by transduction. Thus, we have identified at least 2 genes or gene clusters required for pyoverdine production in P. aeruginosa.