Polysaccharides from the root of Angelica sinensis promotes hematopoiesis and thrombopoiesis through the PI3K/AKT pathway

作者：Chang Liu, Jianqin Li, Fan‐Yi Meng, Simon Xun Liang, Ruixia Deng, Chi Kong Li, N. H. n Pong, Ching Po Lau, Sau Wan Cheng, Jie Yu Ye, Jianliang Chen, S-T Yang, Haixia Yan, Shilin Chen, Beng H. Chong, Mo Yang · 发表于：BMC Complementary and Alternative Medicine · 年份：2010 · DOI：10.1186/1472-6882-10-79 · 被引用次数：115 · 研究领域：Traditional Chinese Medicine Analysis、Platelet Disorders and Treatments、Zebrafish Biomedical Research Applications

BACKGROUND: Dozens of Traditional Chinese Medicine (TCM) formulas have been used for promotion of "blood production" for centuries, and we are interested in developing novel thrombopoietic medicines from these TCMs. Our previous studies have demonstrated the hematopoietic effects of DangGui BuXue Tong (DBT), a formula composed of Radix Angelicae Sinensis and Radix Astragali in animal and cellular models. As a step further to identify and characterize the active chemical components of DBT, we tested the hematopoietic and particularly, thrombopoietic effects of polysaccharide-enriched fractions from the root of Radix Angelicae Sinensis (APS) in this study. METHODS: A myelosuppression mouse model was treated with APS (10 mg/kg/day). Peripheral blood cells from APS, thrombopoietin and vehicle-treated samples were then counted at different time-points. Using the colony-forming unit (CFU) assays, we determined the effects of APS on the proliferation and differentiation of hematopoietic stem/progenitor cells and megakaryocytic lineages. Using a megakaryocytic cell line M-07e as model, we analyzed the cellular apoptosis progression with and without APS treatment by Annexin V, Mitochondrial Membrane Potential and Caspase 3 assays. Last, the anti-apoptotic effect of APS on cells treated with Ly294002, a Phosphatidylinositol 3-Kinse inhibitor (PI3K) was also tested. RESULTS: In animal models, APS significantly enhanced not only the recovery of platelets, other blood cells and their prog...