Interrupted intracarotid artery cold saline infusion as an alternative method for neuroprotection after ischemic stroke

作者：Yabin Ji, Yongming Wu, Zhong Ji, Wei Song, Sui-yi Xu, Yao Wang, Su-Yue Pan · 发表于：Neurosurgical FOCUS · 年份：2012 · DOI：10.3171/2012.5.focus1215 · 被引用次数：38 · 研究领域：Thermal Regulation in Medicine、Acute Ischemic Stroke Management、Neuroinflammation and Neurodegeneration Mechanisms

OBJECT: Intracarotid artery cold saline infusion (ICSI) is an effective method for protecting brain tissue, but its use is limited because of undesirable secondary effects, such as severe decreases in hematocrit levels, as well as its relatively brief duration. In this study, the authors describe and investigate the effects of a novel ICSI pattern (interrupted ICSI) relative to the traditional method (uninterrupted ICSI). METHODS: Ischemic strokes were induced in 85 male Sprague-Dawley rats by occluding the middle cerebral artery for 3 hours using an intraluminal filament. Uninterrupted infusion groups received an infusion at 15 ml/hour for 30 minutes continuously. The same infusion speed was used in the interrupted infusion groups, but the whole duration was divided into trisections, and there was a 20-minute interval without infusion between sections. Forty-eight hours after reperfusion, H & E and silver nitrate staining were utilized for morphological assessment. Infarct sizes and brain water contents were determined using H & E staining and the dry-wet weight method, respectively. Levels of neuron-specific enolase (NSE), S100β protein, and matrix metalloproteinase 9 (MMP-9) in the serum were determined using enzyme-linked immunosorbent assay. Neurological deficits were also evaluated. RESULTS: Histology showed that interrupted ICSI did not affect neurons or fibers in rat brains, which suggests that this method is safe for brain tissues with ischemia. The duration of hypot...