The use of DNA probes identifying restriction‐fragment‐length polymorphisms to examine the Mycobacterium avium complex

作者：Johnjoe McFadden, Philip D. Butcher, J. Thompson, Rodrick J. Chiodini, John Hermon-Taylor · 发表于：Molecular Microbiology · 年份：1987 · DOI：10.1111/j.1365-2958.1987.tb01934.x · 被引用次数：90 · 研究领域：Mycobacterium research and diagnosis、Leprosy Research and Treatment、Tuberculosis Research and Epidemiology

DNA probes were used to identify restriction-fragment-length polymorphisms (RFLPs) in DNA samples, demonstrating that the Mycobacterium avium complex could be clearly divided into M. avium and Mycobacterium intracellulare strains. Less than 2% DNA base substitution was found between M. avium strains, whereas the M. intracellulare strains had greater than 15% base substitution. The Johne's disease bacillus, Mycobacterium paratuberculosis (American type strain), was found to be distinguishable from the M. avium complex serotypes examined. Strain 18 was found to be identical to M. avium. The rat leprosy bacillus, Mycobacterium lepraemurium, was found to be very closely related, but not identical, to M. avium.