Restriction Site Bank Vectors. II. DNA Sequence Analysis of Plasmid pJRD158

作者：Michel Heusterspreute, John Davison · 发表于：DNA · 年份：1984 · DOI：10.1089/dna.1.1984.3.259 · 被引用次数：21 · 研究领域：Molecular Biology Techniques and Applications、Bacteriophages and microbial interactions、RNA and protein synthesis mechanisms

pJRD158 is a small plasmid vector (3903 bp) derived from pBR327 and specifying resistance to ampicillin and tetracycline. It contains 28 unique restriction sites (and 4 nonunique restriction sites) that can be used for cloning. The DNA sequence and computer-assisted restriction site analysis of pJRD158 are reported. Evidence is also presented that suggests a 2-bp revision of the DNA sequence of pBR322 in the RNA primer region.