Formalin-Inactivated EV71 Vaccine Candidate Induced Cross-Neutralizing Antibody against Subgenotypes B1, B4, B5 and C4A in Adult Volunteers

作者：Ai-Hsiang Chou, Chia‐Chyi Liu, Jui-Yuan Chang, Renee Jiang, Yi-Chin Hsieh, Amanda Tsao, Chien-Long Wu, Ju-Lan Huang, Chang-Phone Fung, Szu‐Min Hsieh, Ya Fang Wang, Jen‐Ren Wang, Mei Hu, Jen-Ron Chiang, Ih Jen Su, Pele Choi-Sing Chong · 发表于：PLoS ONE · 年份：2013 · DOI：10.1371/journal.pone.0079783 · 被引用次数：89 · 研究领域：Viral Infections and Immunology Research、Rheumatoid Arthritis Research and Therapies、Animal Disease Management and Epidemiology

BACKGROUND: Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia. No effective EV71 vaccine is available. A randomized and open-label phase I clinical study registered with ClinicalTrials.gov #NCT01268787, aims to evaluate the safety, reactogenicity and immunogenicity of a formalin-inactivated EV71 vaccine candidate (EV71vac) at 5- and 10-µg doses. In this study we report the cross-neutralizing antibody responses from each volunteer against different subgenotypes of EV71 and CVA16. METHODS: Sixty eligible healthy adults were recruited and vaccinated. Blood samples were obtained on day 0, 21 and 42 and tested against B1, B4, B5, C2, C4A, C4B and CVA16 for cross-neutralizing antibody responses. RESULTS: The immunogenicity of both 5- and 10- µg doses were found to be very similar. Approximately 45% of the participants had <8 pre-vaccination neutralization titers (Nt) against the B4 vaccine strain. After the first EV71vac immunization, 95% of vaccinees have >4-fold increase in Nt, but there was no further increase in Nt after the second dose. EV71vac induced very strong cross-neutralizing antibody responses in >85% of volunteers without pre-existing Nt against subgenotype B1, B5 and C4A. EV71vac elicited weak cross-neutralizing antibody responses (∼20% of participants) against a C4B and Coxsackie virus A16. Over 90% of vaccinated volunteers did not develop cross-neutralizing antibody responses (Nt<8) against a C2 strain. EV71vac can b...