Application of a PCR-RFLP Method to Identify Salmon Species in U.S. Commercial Products

作者：Rosalee S. Rasmussen, Michael T. Morrissey, Jessica Walsh · 发表于：Journal of Aquatic Food Product Technology · 年份：2010 · DOI：10.1080/10498850903297576 · 被引用次数：15 · 研究领域：Identification and Quantification in Food、Isotope Analysis in Ecology、Genetic diversity and population structure

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for salmon species identification was optimized for use with U.S. commercial products. Reference specimens of six salmonid species were collected and morphologically verified. A 463- to 464-bp fragment of the mitochondrial tRNAGlu/cytochrome b gene was PCR-amplified, digested with two restriction enzymes (Sau3AI and NlaIII), and analyzed with agarose gel electrophoresis. All six species were successfully differentiated with this method and the restriction digest was shortened to 1 h rather than overnight. A decision-making flowchart was developed based on these results that allows for species diagnosis within two–three steps. After the method was optimized, it was tested with a variety of commercial salmon products (n = 29), including canned, smoked, jerky, and fresh fillet samples. Salmon species identification was successful for all 14 smoked and fresh/frozen fillet products, with the possibility of same-day species diagnosis. Species identification was also achieved for two out of three jerky products, but required overnight lysis. The remainder of the samples could not be diagnosed—including canned salmon, pouch-sterilized salmon, and canned paté. Overall, this method showed high potential for use in same-day species authentication with lightly processed seafood, but heavily processed products will require alternate methods.