Differentiation of Phagocytic Monocytes into Lymph Node Dendritic Cells In Vivo

作者：Gwendalyn J. Randolph, Kayo Inaba, Davide F. Robbiani, Ralph M. Steinman, William A. Müller · 发表于：Immunity · 年份：1999 · DOI：10.1016/s1074-7613(00)80149-1 · 被引用次数：900 · 研究领域：Immunotherapy and Immune Responses、T-cell and B-cell Immunology、Allergic Rhinitis and Sensitization

We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere+ macrophages, which remained in subcutaneous tissue. However, about 25% of latex+ cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.