Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC 25586

作者：Vinayak Kapatral, Iain Anderson, Natalia Ivanova, Gary Reznik, Tamara Los, Athanasios Lykidis, Anamitra Bhattacharyya, Allen E. Bartman, Warren L. Gardner, Galina Grechkin, Lihua Julie Zhu, Olga Vasieva, Lien P. Chu, Yakov N. Kogan, Oleg Y. Chaga, Eugene Goltsman, Axel E. Bernal, Niels Erik Larsen, Mark D’Souza, Theresa L. Walunas, Gordon D. Pusch, Robert Haselkorn, Michael Fonstein, Nikos C. Kyrpides, Ross Overbeek · 发表于：Journal of Bacteriology · 年份：2002 · DOI：10.1128/jb.184.7.2005-2018.2002 · 被引用次数：359 · 研究领域：Oral microbiology and periodontitis research、Otolaryngology and Infectious Diseases、Oral and gingival health research

We present a complete DNA sequence and metabolic analysis of the dominant oral bacterium Fusobacterium nucleatum. Although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens Porphyromonas gingivalis and Bacteroides forsythus. The F. nucleatum strain ATCC 25586 genome was assembled from shotgun sequences and analyzed using the ERGO bioinformatics suite (http://www.integratedgenomics.com). The genome contains 2.17 Mb encoding 2,067 open reading frames, organized on a single circular chromosome with 27% GC content. Despite its taxonomic position among the gram-negative bacteria, several features of its core metabolism are similar to that of gram-positive Clostridium spp., Enterococcus spp., and Lactococcus spp. The genome analysis has revealed several key aspects of the pathways of organic acid, amino acid, carbohydrate, and lipid metabolism. Nine very-high-molecular-weight outer membrane proteins are predicted from the sequence, none of which has been reported in the literature. More than 137 transporters for the uptake of a variety of substrates such as peptides, sugars, metal ions, and cofactors have been identified. Biosynthetic pathways exist for only three amino acids: glutamate, aspartate, and asparagine. The remaining amino acids are imported as such or as di- or oligopeptides that are subsequently degraded in the cytoplasm. A principal source of energy app...