Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS)

作者：Katrin D. Kneipp, Harald Kneipp, V. B. Kartha, Ramasamy Manoharan, Geurt Deinum, Irving Itzkan, Ramachandra Rao Dasari, Michael S. Feld · 发表于：Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics · 年份：1998 · DOI：10.1103/physreve.57.r6281 · 被引用次数：566 · 研究领域：Gold and Silver Nanoparticles Synthesis and Applications、Nanocluster Synthesis and Applications、Advanced biosensing and bioanalysis techniques

Nonresonant Raman cross sections of $\ensuremath{\sim}{10}^{\ensuremath{-}16} {\mathrm{cm}}^{2}$ per molecule are shown to be related to surface-enhanced Raman scattering (SERS) on colloidal silver clusters at near-infrared (NIR) excitation. The enhancement is found to be independent of cluster sizes between 100 nm and 20 \ensuremath{\mu}m. These experimental findings demonstrate that NIR SERS on colloidal silver clusters is an excellent technique for single molecule detection that is applicable for a broad range of molecules including ``colorless'' biomolecules, for example nucleotides in DNA sequencing. As an example, we present the detection of a single adenine molecule without any labeling based on its intrinsic surface-enhanced Raman scattering.