Identification of the structures of multiple subspecies of protein kinase C expressed in rat brain

作者：Ushio Kikkawa, Yoshitaka Ono, Kouji Ogita, Tomoko Fujii, Yoshinori Asaoka, Kazuo Sekiguchi, Yoshiyuki Kosaka, Koichi lgarashi, Yasutomi Nishizuka · 发表于：FEBS Letters · 年份：1987 · DOI：10.1016/0014-5793(87)80668-3 · 被引用次数：200 · 研究领域：Protein Kinase Regulation and GTPase Signaling、Receptor Mechanisms and Signaling、Computational Drug Discovery Methods

Rat brain protein kinase C purified to apparent homogeneity [(1986) Biochem. Biophys. Res. Commun. 135, 636-643] was resolved into three distinct fractions, type I, II and III, upon chromatography on a hydroxyapatite column connected to high-performance liquid chromatography. Comparison of each fraction with the four subspecies of protein kinase C, that were separately expressed in COS cells transfected by the respective cDNAs, alpha, beta I, beta II and d gamma, identified the primary structures of these three fractions of protein kinase C. Type I corresponded to the enzyme encoded by the gamma-sequence; type II was a mixture of the two subspecies determined by the beta I- and beta II-sequences; and type III had the structure encoded by the alpha-sequence. The structures and properties of these subspecies of protein kinase C were similar to each other.