In vivo rabbit hindquarter model for assessment of regional burn hypermetabolism

作者：Ji Xu, Zhewei Fei, Yong‐Ming Yu, Wenyin Xu, Andrew B. Rhodes, Ronald G. Tompkins, John Schulz · 发表于：Journal of Applied Physiology · 年份：2003 · DOI：10.1152/japplphysiol.00513.2002 · 被引用次数：6 · 研究领域：Burn Injury Management and Outcomes、Forensic Entomology and Diptera Studies、Wound Healing and Treatments

Severe burn injury evokes hypermetabolism and muscle wasting, despite nominally adequate nutrition. Although there is much information on whole organism and isolated tissue metabolism after burn injury, data examining regional burn hypermetabolism in vivo are lacking. Using surgically implanted (general anesthesia) regional vascular catheters and primed constant infusion of l-[1-(13)C]phenylalanine tracer, we have determined in vivo burn-induced alterations in rabbit hindquarter protein and energy metabolism. Burn injury evokes increased whole body resting energy expenditure and phenylalanine turnover, accompanied by significantly increased hindquarter proteolysis, creating a negative protein balance in burned rabbit hindquarter. Hindquarter oxygen consumption showed an increase after burn injury, but it did not reach statistical significance. Burn-induced changes in hindquarter protein turnover account for approximately one-third of the whole animal hypermetabolism. This model offers a system for regional manipulation of postburn hypermetabolism.