Embryonic Stem Cell Lines Derived from Human Blastocysts

作者：James A. Thomson, Joseph Itskovitz‐Eldor, Sander S. Shapiro, Michelle Waknitz, Jennifer J. Swiergiel, Vivienne S. Marshall, Jeffrey M. Jones · 发表于：Science · 年份：1998 · DOI：10.1126/science.282.5391.1145 · 被引用次数：16010 · 研究领域：Pluripotent Stem Cells Research、Mesenchymal stem cell research、Tissue Engineering and Regenerative Medicine

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.