Regulation of Interleukin-2 Gene Enhancer Activity by the T Cell Accessory Molecule CD28

作者：James D. Fraser, Bryan A. Irving, Gerald R. Crabtree, Arthur Weiss · 发表于：Science · 年份：1991 · DOI：10.1126/science.1846244 · 被引用次数：649 · 研究领域：Immune Cell Function and Interaction、T-cell and B-cell Immunology、NF-κB Signaling Pathways

The mechanism by which cell surface molecules regulate T cell production of lymphokines is poorly understood. Production of interleukin-2 (IL-2) can be regulated by signal transduction pathways distinct from those induced by the T cell antigen receptor. Stimulation of CD28, a molecule expressed on most human T cells, induced the formation of a protein complex that bound to a site on the IL-2 gene distinct from previously described binding sites and increased IL-2 enhancer activity fivefold. The CD28-responsive complex bound to the IL-2 gene between -164 and -154 base pairs from the transcription start site. The sequence of this element is similar to regions conserved in the 5' flanking regions of several other lymphokine genes.